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• The study population from which the sample is drawn should be representative of the population in which the test is to be applied;
• The sample of individuals to which the test is applied must be selected in a manner to ensure that it is representative of the study population;
• The sample should include animals in all stages of the infection/disease process;
• The sample size must be sufficient to provide adequate precision (confidence limits) about the estimate; and
• Testing should be undertaken with blinding as to the true status of the individual and to other test results.