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Results for animals that are disease free (D-) usually overlap with the results in the diseased population (D+). Animals to the right of the cut-point (C-C) are classified as reactors (diseased or infected) and animals to the left are classified as negative (non-infected). If fewer false positives are required, C-C is moved to the right; specificity increases and sensitivity decreases. However, if fewer false negatives are required, C-C is moved to the left: sensitivity increases and specificity decreases.