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During the Menangle virus investigations, a wide range of additional investigations were undertaken, including:

• Detailed pathological, serological, microbiological and virological examination of affected and unaffected pigs to determine the likely cause and to rule out known infections and other diseases.
• Cross-sectional serological survey of all units/sheds to determine the extent and progress of infection.
• Surveys of pigs and piggeries in contact to determine whether infection had spread beyond the Menangle piggery.
• Sampling of unexposed piggeries to demonstrate freedom of the rest of the industry.
• Testing of archived sera (from this and other piggeries nation-wide) to demonstrate that it was a new infection not previously present in the Australian pig population.
• Interview and testing of piggery workers and others potentially exposed to evaluate public health risks.
• Serology on other species as potential sources.
• Serology and virus isolation on fruit bats to support the hypothesis that they were the likely source.}}