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There are a range of issues relating to selection of animals for a cohort study as well. Animals eligible for inclusion in a cohort study should be free of the disease of interest at the start of the study and should be at risk of developing the disease. If animals may have previously had the disease and recovered and are now immune then these animals are no longer at risk of getting the disease and should be excluded from selection. Other diseases may recur once some time has elapsed for recovery. In these situations it may be possible to state that animals become eligible for inclusion in the study providing they have not had the disease within a defined period prior to the start of the study.